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Kit Deteksi Kanker Leher Rahim Berbasis Antibodi Telomerase: Pendekatan secara Immunomolekular

机译:基于端粒酶抗体的宫颈癌检测试剂盒:一种免疫分子方法

摘要

Cervical cancer is the first leading cause of cancer death in Indonesia. About 95% telomerase activity is associated with cervical cancer\u27s Malignancy. Therefore, telomerase is very potential as a biomarker for cervical cancer detection. This research\u27s purpose is to produce cut-off point of cervical cancer telomerase. Telomerase antibody is produced and detected using Western Blotting and ELISA, 15 samples of each cancer and normal patients were collected from RSSA. ELISA Absorbance value of antibody and antigen binding were collected and analyzed using independent t-test and ROC. cut off point is found at 0.092 with 81,25% specificity and 80% sensitivity.
机译:宫颈癌是印度尼西亚癌症死亡的第一大主要原因。约95%的端粒酶活性与宫颈癌恶性程度有关。因此,端粒酶作为检测宫颈癌的生物标志物非常有潜力。这项研究的目的是产生宫颈癌端粒酶的临界值。使用Western Blotting和ELISA产生并检测端粒酶抗体,从RSSA收集每种癌症和正常患者的15个样品。 ELISA收集抗体的吸光度值和抗原结合,并使用独立的t检验和ROC进行分析。截止点为0.092,特异性为81,25%,灵敏度为80%。

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